![]() The RNA molecules are then transferred onto a nylon membrane and then fixed using heat or UV radiation. The RNA molecules are separated by a special type of electrophoresis which involves the use of denaturing agent formaldehyde along with the agarose gel. Specific RNA sequences are detected using this technique. The membrane is then treated with UV (ultraviolet) radiation for the permanent attachment of the DNA molecules on the membrane surface then the membrane is treated with specific labeled DNA probes which are complementary and bind to the DNA and help in their detection. The separated DNA fragments are then transferred onto a nitrocellulose membrane by placing the membrane over the gel and applying pressure so that the DNA molecules get attached to the membrane. The fragments are then separated with respect to their size on a gel by the method of electrophoresis. The DNA molecules are digested into smaller fragments by using specific enzymes called restriction endonucleases. Specific DNA sequences are identified using this blotting technique. Southern blotting is used for the detection of DNA molecules, northern blotting is used for RNA detection and western blotting is used for the detection of proteins. The blotting techniques come in a variety of forms, the three main ones being southern, northern, and western blotting. In this technique, the macromolecules under investigation are first separated on a gel matrix through another process called electrophoresis, and then the resolved components are transferred onto nitrocellulose or any other suitable membrane and their detection is carried out using a small in-vitro designed RNA (ribonucleic acids) or DNA (deoxyribonucleic acids) which have complementarity with the molecule under assessment and are called probes. Australian Financial Accounting (Craig Deegan)īlotting is a technique extensively used in the field of molecular biology to distinguish different nucleic acids (DNA and RNA) and proteins.Financial Institutions, Instruments and Markets (Viney Michael McGrath Christopher Viney).Lawyers' Professional Responsibility (Gino Dal Pont).Contract: Cases and Materials (Paterson Jeannie Robertson Andrew Duke).Culture and Psychology (Matsumoto David Matsumoto Linda Juang). ![]()
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